Composite

Part:BBa_K4340600:Design

Designed by: Chein Yueh Liu   Group: iGEM22_PuiChing_Macau   (2022-09-29)


T2R4 with NSP4 peptides


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 346
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We firstly searched hormone binding proteins to target reducing the effect of hormone that stops or delays seed germination. We found that T2R4 is suitable for inhibiting ABA, however, the efficiency is not very high. Therefore, we added NSP4 secretion peptides before it to help with its efficiency.


Source

BBa_K3852004 for T2R4 BBa_K2760000 for NSP4


References